DRUG DISCOVERY & DEVELOPMENT

DRUG DISCOVERY & DEVELOPMENT Presents an Educational Webcast

11:00 a.m. ET; 8:00 a.m. PT • Wednesday, August 28, 2013 • Duration: 1 Hour

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Bioluminescence

Webcast Spotlight

resonance energy

Join us and learn about:

transfer has been long established as

• Promega's novel NanoBRET™

the principal

technology using NanoLuc™

method to monitor

and HaloTag ®

dynamic protein-

• Application of NanoBRET™

protein interactions

for measuring protein-protein

in living cells. However, the

interactions in living cells

application of BRET

• The new CLARIOstar ® high

is in general

performance multimode

hampered by its

microplate reader

limited dynamic range due to the

• The revolutionary new LVF

poor spectral

Monchromator™ from BMG

separation of donor

LABTECH

and acceptor. In

• Wavelength scanning in

this webcast we will

luminescence assays

introduce

• How to enhance all of your

NanoBRET™ using a donor/accpetor

assays with the CLARIOstar ®

combination

microplate reader.

consisting of NanoLuc™ luciferase and Halotag ® labeling technology.

The superior brightness of NanoLuc™ makes it ideally suited as an energy donor for BRET applications and allows the use of a novel red-shifted Halotag ® ligand as acceptor. The resulting increase in spectral separation between donor and acceptor translates into significantly improved dynamic range compared to current Rluc/YFP based BRET technology, which makes NanoBRET™ more suitable for the development of HTS compatible cell based protein-protein interaction assays.

For the NanoLuc ® BRET assay, two emission signals need to be measured at two different wavelength ranges. To get the best performance in this assay, wider bandwidths are preferred. Therefore, microplate readers that used filters with wider bandwidths always performed better in this assay than those instruments that used grating-based monochromators, which have fixed or adjustable bandwidths of < 25nm.

Until Now.

This webcast will highlight the performance of the NanoLuc ® BRET Assay from Promega on the new CLARIOstar ® multimode microplate reader from BMG LABTECH. Among many other great features, the CLARIOstar ® has a revolutionary new type of LVF Monochromator™ that has adjustable bandwidths from 8 to 100 nm.

PANELISTS MODERATOR

Tim Studt

E.J. Dell, Ph.D.

Thomas

Editorial Director

International

Machleidt, Ph.D.

Drug Discovery &

Marketing Director

Head of Cell

BMG LABTECH,

Biology,

Development

GmbH

Advanced Technology Group Promega Corporation

REMINDER: Attend this live webcast and pose your critical questions to the panel of experts during the live Q&A session during the broadcast.

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