DRUG DISCOVERY & DEVELOPMENT Presents an Educational Webcast
11:00 a.m. ET; 8:00 a.m. PT • Wednesday, August 28, 2013 • Duration: 1 Hour
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Bioluminescence
Webcast Spotlight
resonance energy
Join us and learn about:
transfer has been long established as
• Promega's novel NanoBRET™
the principal
technology using NanoLuc™
method to monitor
and HaloTag ®
dynamic protein-
• Application of NanoBRET™
protein interactions
for measuring protein-protein
in living cells. However, the
interactions in living cells
application of BRET
• The new CLARIOstar ® high
is in general
performance multimode
hampered by its
microplate reader
limited dynamic range due to the
• The revolutionary new LVF
poor spectral
Monchromator™ from BMG
separation of donor
LABTECH
and acceptor. In
• Wavelength scanning in
this webcast we will
luminescence assays
introduce
• How to enhance all of your
NanoBRET™ using a donor/accpetor
assays with the CLARIOstar ®
combination
microplate reader.
consisting of NanoLuc™ luciferase and Halotag ® labeling technology.
The superior brightness of NanoLuc™ makes it ideally suited as an energy donor for BRET applications and allows the use of a novel red-shifted Halotag ® ligand as acceptor. The resulting increase in spectral separation between donor and acceptor translates into significantly improved dynamic range compared to current Rluc/YFP based BRET technology, which makes NanoBRET™ more suitable for the development of HTS compatible cell based protein-protein interaction assays.
For the NanoLuc ® BRET assay, two emission signals need to be measured at two different wavelength ranges. To get the best performance in this assay, wider bandwidths are preferred. Therefore, microplate readers that used filters with wider bandwidths always performed better in this assay than those instruments that used grating-based monochromators, which have fixed or adjustable bandwidths of < 25nm.
Until Now.
This webcast will highlight the performance of the NanoLuc ® BRET Assay from Promega on the new CLARIOstar ® multimode microplate reader from BMG LABTECH. Among many other great features, the CLARIOstar ® has a revolutionary new type of LVF Monochromator™ that has adjustable bandwidths from 8 to 100 nm.
PANELISTS MODERATOR
Tim Studt
E.J. Dell, Ph.D.
Thomas
Editorial Director
International
Machleidt, Ph.D.
Drug Discovery &
Marketing Director
Head of Cell
BMG LABTECH,
Biology,
Development
GmbH
Advanced Technology Group Promega Corporation
REMINDER: Attend this live webcast and pose your critical questions to the panel of experts during the live Q&A session during the broadcast.
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